Late Iron Age necropolis of Seminario Vescovile in Verona (Northern Italy, 3rd-1st).

[Stefano]

I managed to convert 2 of the BAM files to 23andme raw data format using WGSExtract.
Unfortunately after i restarted the PC today, for some reason the program does not work anymore. it keeps saying "INVALID HEADER" when selecting the BAM files. i even tried to re-download them.
If someone wants to try here is the installer https://wgsextract.github.io/
and here the BAMs https://www.ebi.ac.uk/ena/browser/view/P...ed-records
So now, one of the 2 raw data i got is really small and low coverage, the other is decent.

Here it is
https://jumpshare.com/s/xMdkKOnmMSKkhaokUbLk

Simulated g25 from raw data using this https://www.exploreyourdna.com/rawtosimg25.aspx
CisalpineGaul_simulated_g25_scaled,0.12431,0.148961,0.041693,-0.000243,0.04654,-0.002854,0.000567,0.003719,0.019368,0.030393,-0.002852,0.005666,-0.0108,0.001443,-0.00644,-0.003143,0.003952,-0.000379,0.00322,-0.005928,-0.003865,-0.002557,0.000787,-0.004603,-0.001261

TUR_Barcin_N: 58.61 %
Yamnaya_RUS_Samara: 29.58 %
WHG: 11.62 %
MAR_Taforalt: 0.19 %

Distance to: CisalpineGaul_simulated_g25_scaled
0.02072854 Italian_Trentino-Alto-Adige
0.02330942 Spanish_Menorca
0.02344690 Spanish_Mallorca
0.02350668 Spanish_Baleares
0.02427447 Spanish_Peri-Barcelona
0.02472052 Italian_Veneto
0.02529940 Spanish_Catalunya_Central
0.02538017 Spanish_Girona
0.02556982 Spanish_Barcelones
0.02578240 Italian_Bergamo
0.02634774 Spanish_Pirineu
0.02645921 Spanish_Terres_de_l'Ebre
0.02648412 Spanish_Eivissa
0.02649972 Spanish_Camp_de_Tarragona
0.02710578 Italian_Northeast
0.02751564 Spanish_Lleida
0.02767254 Spanish_Penedes
0.02811645 Spanish_Valencia
0.02834991 Spanish_Cataluna
0.02952521 Spanish_Cantabria
0.02958048 Spanish_Castello
0.02961410 Spanish_Murcia
0.03020494 Spanish_Castilla_La_Mancha
0.03045957 French_Auvergne
0.03078674 French_Provence


Distance to: CisalpineGaul_simulated_g25_scaled
0.01757812 SRB_BA
0.01828934 HRV_IA
0.01945620 CZE_LBA_Knoviz_o3
0.02279324 AUT_IA_La_Tene
0.02337043 SVN_EIA
0.02350412 HRV_EIA
0.02386863 HUN_La_Tene
0.02455299 ITA_Etruscan_Casenovole
0.02490596 HUN_MBA_Vatya
0.02504431 HUN_IA_La_Tene
0.02605650 HRV_LIA_La_Tene
0.02647629 SRB_Mokrin_EBA_Maros
0.02701471 HRV_Bezdanjaca_BA
0.02705574 DEU_Roman
0.02729670 ITA_Etruscan
0.02756998 Iberia_Northeast_c.8-12CE
0.02781036 SRB_BA_Maros
0.02796691 ITA_Etruscan_Volterrra
0.02817529 HRV_BA
0.02890016 HRV_MBA
0.03018228 ITA_Etruscan_Tarquinia
0.03021870 CHE_IA
0.03076353 ITA_Ardea_Latini_IA
0.03145492 SVK_IA_Vekerzug
0.03203831 SRB_Mokrin_EBA_Maros_oAegean

[R.Rocca]

I had the same problem when the files came out using SAMTOOLS.

[Stefano]

Problem solved! My antivirus mistakenly removed an .exe in the WGSExtract program, making the error message appear. Over the next weeks i will slowly convert more files.
Here is another one, with higher coverage.

https://jumpshare.com/v/awnqLMmXcjZnuCzxWGA5

CisalpineGaul3217,0.12462,0.135,0.04808,0.020314,0.046234,0.009448,0.00341,0.005566,0.010927,0.014739,-0.003288,0.002175,-0.009305,0.007834,0.000204,0.003298,0.004598,0.000308,0.004512,0.002527,0.002543,-0.006572,0.000572,-0.00035,0.002245

TUR_Barcin_N: 48.38 %
Yamnaya_RUS_Samara: 37.50 %
WHG: 13.52 %
Han: 0.60 %

Distance to: CisalpineGaul3217
0.02251143 SVK_IA_Vekerzug
0.02329088 HUN_EIA_Prescythian_Mezocsat
0.02332543 HUN_IA_La_Tene
0.02407875 Iberia_Northeast_c.6CE_PL
0.02612487 HUN_Sarmatian_Late_Transtisza
0.02632440 ITA_Rome_Renaissance
0.02646192 SRB_Mokrin_EBA_Maros
0.02704047 SVK_LIA_La_Tene
0.02711580 CZE_IA_Hallstatt
0.02886671 SRB_BA_Maros
0.02945381 HUN_LBA
0.02977671 CZE_IA_La_Tene_low_res
0.02980453 HUN_MA_Szolad
0.02990001 Scythian_HUN
0.03020432 DEU_Lech_EBA_contam
0.03090333 CZE_LBA_Knoviz
0.03131209 CZE_IA_La_Tene
0.03153138 CZE_MBA_Tumulus
0.03153908 AUT_IA_La_Tene
0.03193941 HRV_Bezdanjaca_BA
0.03198100 HUN_EIA_Hallstatt
0.03250646 ITA_Collegno_MA
0.03279358 ITA_Etruscan_Vetulonia_CEU_o
0.03311274 ITA_Etruscan_Campiglia_CEU_o
0.03443364 HUN_IA_La_Tene_possible



Distance to: CisalpineGaul3217
0.02435582 French_Alsace
0.02731995 Austrian
0.02760215 Swiss_German
0.02789802 French_Nord
0.02955400 French_Auvergne
0.03017291 BelgianC
0.03019621 French_Occitanie
0.03167950 BelgianB
0.03185122 Swiss_French
0.03189019 French_Paris
0.03199863 German_Erlangen
0.03210706 Italian_Northeast
0.03234809 Montenegrin
0.03285703 French_Pas-de-Calais
0.03440708 German
0.03464071 French_Provence
0.03494773 BelgianA
0.03505965 Croatian
0.03554401 French_Seine-Maritime
0.03556823 Romanian
0.03559967 Slovenian
0.03610214 Serbian
0.03622286 Italian_Aosta_Valley
0.03645201 Hungarian
0.03699711 Bosnian


Distance to: CisalpineGaul3217
0.02540033 Alpine_Celts
0.02596216 Boii
0.02862560 Cisalpine_Gauls
0.02882157 Celts
0.03552938 Belgae
0.03752349 Iazyges
0.04334796 Iapodes
0.04397368 Transalpine_Gauls
0.04847124 England_Celts
0.05339472 Baiuvari
0.05364192 Pannonii
0.05439139 Etruscans
0.05778635 Alemanni
0.05827046 Vascones
0.06161154 Saxons
0.06492301 Picts
0.06806043 Gothones
0.06931791 Longobards
0.07036860 Illyrians
0.07082284 Scandza
0.09446140 Romans
0.09771986 Slavs
0.12075972 Magna_Graecia
0.13054180 Roman_Greeks
0.13104673 Balti

[Fabrice E]

thanks for your share Stepano

is it a sim or real G25 from David ?

[ChrisR]

Thx @Stefano - I renamed your samples and together with one Rhaetia-Roman-Late:Burgeis combined them into my Euro PCA sample selection

Code:

Rhaetia-Roman-Late:Burgeis-c500AD-BSS8-US113-2069_Y-E_K12bsimAL,0.110830,0.165466,0.028774,-0.012953,0.040939,-0.009695,0.008374,0.012928,0.011073,0.027726,-0.001510,0.005859,-0.010645,-0.004469,0.001736,-0.002219,-0.000278,-0.000183,-0.000234,0.000658,0.002113,0.000971,-0.002772,-0.001500,-0.000003
Cenomani-Cisalp-Gaul:Verona-2cBC_3217-RawSimEyDSc,0.12462,0.135,0.04808,0.020314,0.046234,0.009448,0.00341,0.005566,0.010927,0.014739,-0.003288,0.002175,-0.009305,0.007834,0.000204,0.003298,0.004598,0.000308,0.004512,0.002527,0.002543,-0.006572,0.000572,-0.00035,0.002245
Cenomani-Cisalp-Gaul:Verona-2cBC_LQ-RawSimEyDSc,0.12431,0.148961,0.041693,-0.000243,0.04654,-0.002854,0.000567,0.003719,0.019368,0.030393,-0.002852,0.005666,-0.0108,0.001443,-0.00644,-0.003143,0.003952,-0.000379,0.00322,-0.005928,-0.003865,-0.002557,0.000787,-0.004603,-0.001261

The left one is the LQ (low res) one and the right one the second one Stefano posted. Would be interesting to have more Cenomani and as well Rhaetia results. I have not checked if Davidski has yet included official G25 for (some) of the Rhaetia results. PS: Capitals are official G25 (averages), the other are "my sample selection".

[Dewsloth]

Thx @Stefano - I renamed your samples and together with one Rhaetia-Roman-Late:Burgeis combined them into my Euro PCA sample selection

Code:

Rhaetia-Roman-Late:Burgeis-c500AD-BSS8-US113-2069_Y-E_K12bsimAL,0.110830,0.165466,0.028774,-0.012953,0.040939,-0.009695,0.008374,0.012928,0.011073,0.027726,-0.001510,0.005859,-0.010645,-0.004469,0.001736,-0.002219,-0.000278,-0.000183,-0.000234,0.000658,0.002113,0.000971,-0.002772,-0.001500,-0.000003
Cenomani-Cisalp-Gaul:Verona-2cBC_3217-RawSimEyDSc,0.12462,0.135,0.04808,0.020314,0.046234,0.009448,0.00341,0.005566,0.010927,0.014739,-0.003288,0.002175,-0.009305,0.007834,0.000204,0.003298,0.004598,0.000308,0.004512,0.002527,0.002543,-0.006572,0.000572,-0.00035,0.002245
Cenomani-Cisalp-Gaul:Verona-2cBC_LQ-RawSimEyDSc,0.12431,0.148961,0.041693,-0.000243,0.04654,-0.002854,0.000567,0.003719,0.019368,0.030393,-0.002852,0.005666,-0.0108,0.001443,-0.00644,-0.003143,0.003952,-0.000379,0.00322,-0.005928,-0.003865,-0.002557,0.000787,-0.004603,-0.001261

The left one is the LQ (low res) one and the right one the second one Stefano posted. Would be interesting to have more Cenomani and as well Rhaetia results. I have not checked if Davidski has yet included official G25 for (some) of the Rhaetia results. PS: Capitals are official G25 (averages), the other are "my sample selection".

I'm curious how the WEuro Klosterneuburg auxiliaries would plot on this.  They could be as early as 25 CE and one (R10656) is L21 of unknown (Isles or Continent?) origin.  The other two are DF19 and U106 (a neonate whose father was probably the auxiliary, mother local?).

Austria_Klosterneuburg_Roman.SG:R10656.SG,0.138864,0.126941,0.069013,0.057494,0.037238,0.011435,0.002585,0.008769,0.002454,0.006196,0.000487,0.006744,-0.023934,-0.010046,0.019408,0.012729,0.001434,-0.001394,-0.001006,0.001251,0.003244,0.000247,0.006779,0.010122,0.006586
Austria_Klosterneuburg_Roman.SG:R10657.SG,0.124067,0.128972,0.060339,0.073967,0.029852,0.019801,0.00188,0.006692,0.005318,-0.004556,-0.009743,0.004946,-0.011744,-0.009496,0.02348,0.010475,-0.010952,0.002407,0.004148,0.004502,0.000998,0.002597,-0.004314,0.019521,0.003473
Austria_Klosterneuburg_Roman.SG:R10659.SG,0.135449,0.135065,0.067882,0.02907,0.0437,0.012829,0.001645,0.003461,0.016566,0.007107,-0.001299,0.000899,-0.007582,-0.007156,0.014251,0.004375,-0.001956,-0.000127,0.005908,0.007879,0.008735,0.000742,0.000246,0.006145,-0.001437

[ChrisR]

I forgot to ask: do we have other Late IE Gaul or Noric G25 coords? Are some of the La-Tene samples good to use? The samples I used are all North and West of ALSACE so the Cisalpine Cenomani from this study seem already pretty shifted shouthwards, more then even (earlier) Etruscans and Latins, it seems similar to Rheatians - but we would like to have pre-Roman Rhaetians to confirm this.

[ChrisR]

I'm curious how the WEuro Klosterneuburg auxiliaries would plot on this.  They could be as early as 25 CE and one (R10656) is L21 of unknown (Isles or Continent?) origin.  The other two are DF19 and U106 (a neonate whose father was probably the auxiliary, mother local?).

They plot similarly to Hungarian Langobards (also similar to Saxons, Vikings, etc.) and are way North of GERMAN so would not even plot on this zoom selection

[Mitchell-Atkins]

I had the same problem when the files came out using SAMTOOLS.

Wonder if your solution is similar to stefano’s…antivirus software issue

“ Problem solved! My antivirus mistakenly removed an .exe in the WGSExtract program, making the error message appear. Over the next weeks i will slowly convert more files.”

https://genarchivist.com/showthread.php?...8#pid12418

[Moeca]

Problem solved! My antivirus mistakenly removed an .exe in the WGSExtract program, making the error message appear. Over the next weeks i will slowly convert more files.
Here is another one, with higher coverage.

https://jumpshare.com/v/awnqLMmXcjZnuCzxWGA5

CisalpineGaul3217,0.12462,0.135,0.04808,0.020314,0.046234,0.009448,0.00341,0.005566,0.010927,0.014739,-0.003288,0.002175,-0.009305,0.007834,0.000204,0.003298,0.004598,0.000308,0.004512,0.002527,0.002543,-0.006572,0.000572,-0.00035,0.002245

TUR_Barcin_N: 48.38 %
Yamnaya_RUS_Samara: 37.50 %
WHG: 13.52 %
Han: 0.60 %

Distance to: CisalpineGaul3217
0.02251143 SVK_IA_Vekerzug
0.02329088 HUN_EIA_Prescythian_Mezocsat
0.02332543 HUN_IA_La_Tene
0.02407875 Iberia_Northeast_c.6CE_PL
0.02612487 HUN_Sarmatian_Late_Transtisza
0.02632440 ITA_Rome_Renaissance
0.02646192 SRB_Mokrin_EBA_Maros
0.02704047 SVK_LIA_La_Tene
0.02711580 CZE_IA_Hallstatt
0.02886671 SRB_BA_Maros
0.02945381 HUN_LBA
0.02977671 CZE_IA_La_Tene_low_res
0.02980453 HUN_MA_Szolad
0.02990001 Scythian_HUN
0.03020432 DEU_Lech_EBA_contam
0.03090333 CZE_LBA_Knoviz
0.03131209 CZE_IA_La_Tene
0.03153138 CZE_MBA_Tumulus
0.03153908 AUT_IA_La_Tene
0.03193941 HRV_Bezdanjaca_BA
0.03198100 HUN_EIA_Hallstatt
0.03250646 ITA_Collegno_MA
0.03279358 ITA_Etruscan_Vetulonia_CEU_o
0.03311274 ITA_Etruscan_Campiglia_CEU_o
0.03443364 HUN_IA_La_Tene_possible



Distance to: CisalpineGaul3217
0.02435582 French_Alsace
0.02731995 Austrian
0.02760215 Swiss_German
0.02789802 French_Nord
0.02955400 French_Auvergne
0.03017291 BelgianC
0.03019621 French_Occitanie
0.03167950 BelgianB
0.03185122 Swiss_French
0.03189019 French_Paris
0.03199863 German_Erlangen
0.03210706 Italian_Northeast
0.03234809 Montenegrin
0.03285703 French_Pas-de-Calais
0.03440708 German
0.03464071 French_Provence
0.03494773 BelgianA
0.03505965 Croatian
0.03554401 French_Seine-Maritime
0.03556823 Romanian
0.03559967 Slovenian
0.03610214 Serbian
0.03622286 Italian_Aosta_Valley
0.03645201 Hungarian
0.03699711 Bosnian


Distance to: CisalpineGaul3217
0.02540033 Alpine_Celts
0.02596216 Boii
0.02862560 Cisalpine_Gauls
0.02882157 Celts
0.03552938 Belgae
0.03752349 Iazyges
0.04334796 Iapodes
0.04397368 Transalpine_Gauls
0.04847124 England_Celts
0.05339472 Baiuvari
0.05364192 Pannonii
0.05439139 Etruscans
0.05778635 Alemanni
0.05827046 Vascones
0.06161154 Saxons
0.06492301 Picts
0.06806043 Gothones
0.06931791 Longobards
0.07036860 Illyrians
0.07082284 Scandza
0.09446140 Romans
0.09771986 Slavs
0.12075972 Magna_Graecia
0.13054180 Roman_Greeks
0.13104673 Balti

I could not open your link..........................can you pull these out for myself

 Alpine_Celts
 Boii
Cisalpine_Gauls
 Celts
 Belgae
Iazyges
 Iapodes
Transalpine_Gauls


but I did run some against myself , see below

Distance to: Moeca_scaled
0.02098513 Liburnian_G25_scaled
0.03531488 Cenomani-Cisalp-Gaul:Verona-2cBC_LQ-RawSimEyDSc
0.04000736 Rhaetia-Roman-Late:Burgeis-c500AD-BSS8-US113-2069_Y-E_K12bsimAL
0.04774205 Cenomani-Cisalp-Gaul:Verona-2cBC_3217-RawSimEyDSc
0.06801038 Austria_Klosterneuburg_Roman.SG:R10659.SG
0.09190853 Austria_Klosterneuburg_Roman.SG:R10656.SG
0.09966510 Austria_Klosterneuburg_Roman.SG:R10657.SG
0.12676167 ITA_Remedello_BA
0.37858301 ITA_Villabruna

[ChrisR]

...

Distance to: CisalpineGaul3217
0.02540033 Alpine_Celts
0.02596216 Boii
0.02862560 Cisalpine_Gauls
...

Have not inspected the third run enough and would also be interested: is this a public model? Can the used samples be shared?
So far I was not aware results for example were available for Boii, Baiuvari.

[cheshire]

Code:

Italy_Verona_IA:001_sim,0.12431,0.148961,0.041693,-0.000243,0.04654,-0.002854,0.000567,0.003719,0.019368,0.030393,-0.002852,0.005666,-0.0108,0.001443,-0.00644,-0.003143,0.003952,-0.000379,0.00322,-0.005928,-0.003865,-0.002557,0.000787,-0.004603,-0.001261

Code:

Italy_Verona_IA:002_sim,0.12462,0.135,0.04808,0.020314,0.046234,0.009448,0.00341,0.005566,0.010927,0.014739,-0.003288,0.002175,-0.009305,0.007834,0.000204,0.003298,0.004598,0.000308,0.004512,0.002527,0.002543,-0.006572,0.000572,-0.00035,0.002245

[cheshire]

Taurisci: AUT_IA/La_Tene + HUN_IA/La_Tene_West
Carni: SVN_IA

[cheshire]

The first sample is perfectly within the cline CHE_IA ~ ITA_IA, the second one is more central european celt (east of rhine) like, which is similar to another leaked (allegedly) cenomani sample I have.



Northern_Italy_IA?

Code:

Northern_Italy_IA?:DEU_Roman:FN_2__AD_300__Cov_91.96%,0.117238,0.151314,0.045254,0.000323,0.052317,0.001952,0.0047,-0.006,0.01943,0.028064,-0.002436,0.004946,-0.017393,-0.013212,-0.004614,0.00411,0.009388,0.006334,-0.000377,0.0005,0.000624,0.001484,-0.000616,-0.00735,-0.001796
Northern_Italy_IA?:CHE_IA:SX18__BC_82__Cov_52.40%,0.125205,0.153345,0.050534,-0.003876,0.049548,-0.005578,0.0047,-0.002077,0.014726,0.033896,0.001786,0.016335,-0.016353,-0.008945,0.001764,-0.001591,0.009518,0.001647,0.002514,-0.006128,0.005241,0.005688,-0.012078,-0.004338,0.000958
Northern_Italy_IA?:ITA_Etruscan_Casenovole:CSN009__BC_100__Cov_22.26%,0.135449,0.151314,0.047517,-0.001292,0.045547,0.004741,0.005875,-0.002077,0.010226,0.026424,0.003248,0.004646,-0.015461,-0.008945,0,-0.005304,0.013038,-0.0019,0.008673,-0.002001,0.003743,-0.003462,0.003821,-0.000602,-0.004071

[Stefano]

thanks for your share Stepano

is it a sim or real G25 from David ?

Simulated